Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HeLa using 66493-1-Ig
WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells.
WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells.
WB analysis using 66493-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 66493-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66493-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 66493-1-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 66493-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 66493-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66493-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human lung cancer using 66493-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HeLa using 66493-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
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human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human lung cancer tissue
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:150-1:600
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.
Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HeLa using 66493-1-Ig
WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells.
WB analysis using 66493-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 66493-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66493-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 66493-1-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 66493-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human lung cancer using 66493-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66493-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of human lung cancer using 66493-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
IF/ICC Figures
IF Staining of HeLa using 66493-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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