PKNOX1 Polyclonal antibody

PKNOX1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10614-1-AP

Synonyms

Homeobox protein PKNOX1, Homeobox protein PREP 1, PBX/knotted 1 homeobox 1, PBX/knotted homeobox 1, PKNOX1, pkonx1c, PREP1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 10614-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10614-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10614-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 10614-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10614-1-AP (PKNOX1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HepG2 using 10614-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10614-1-AP (PKNOX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, COLO 320 cells, HepG2 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

10614-1-AP targets PKNOX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKNOX1 fusion protein Ag0939
• Full Name: PBX/knotted 1 homeobox 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007746
• Gene Symbol: PKNOX1
• Gene ID (NCBI): 5316
• RRID: AB_2164125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Am J Hum Genet; Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities.; Authors - Noriko Miyake
mouse	WB,IHC	Am J Physiol Renal Physiol; PMID: 31091119; Authors - Zhiqiang Chen