PLA2G1B Monoclonal antibody, PBS Only (Capture)

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  WB analysis of pig pancreas using 66397-1-Ig (same clone as 66397-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66397-1-PBS in a different storage buffer formulation.

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  Sample test of MP51376-1

  Sample test of MP51376-1, PLA2G1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66397-1-PBS. Detection antibody: 66397-2-PBS.

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  IHC staining of human pancreas using 66397-1-Ig (same clone as 66397-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66397-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66397-1-Ig (same clone as 66397-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66397-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51376-1

  Cytometric bead array standard curve of MP51376-1, PLA2G1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66397-1-PBS. Detection antibody: 66397-2-PBS. Standard:Ag8812. Range: 0.391-400 ng/mL.

PLA2G1B Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 66397-1-PBS

Clone No.1F2C10

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

1F2C10, EC:3.1.1.4, Phosphatidylcholine 2-acylhydrolase 1B, PLA2

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Product Information

66397-1-PBS targets PLA2G1B as part of a matched antibody pair:

MP51376-1: 66397-1-PBS capture and 66397-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLA2G1B fusion protein Ag8812
• Full Name: phospholipase A2, group IB (pancreas)
• Calculated Molecular Weight: 70aa,8 kDa; 148aa,16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC005386
• Gene Symbol: PLA2G1B
• Gene ID (NCBI): 5319
• RRID: AB_2881771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04054
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.