PLA2G4E Polyclonal antibody, PBS Only

• 

  WB analysis of SH-SY5Y using 18088-1-AP (same clone as 18088-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SW 1990 using 18088-1-AP (same clone as 18088-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 18088-1-AP (same clone as 18088-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PLA2G4E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18088-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

phospholipase A2, group IVE, PLA2G4E Ens

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Product Information

18088-1-PBS targets PLA2G4E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12808

  Product name: Recombinant human PLA2G4E protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 519-868 aa of BC101584

  Sequence: KDDVSNQDFREWFEFSPYEVGLQKYGAFIPSELFGSEFFMGRLVKRIPESRICYMLGLWSSIFSLNLLDAWNLSHTSEEFFHRWTREKVQDIEDEPILPEIPKCDANILETTVVIPGSWLSNSFREILTHRSFVSEFHNFLSGLQLHTNYLQNGQFSRWKDTVLDGFPNQLTESANHLCLLDTAFFVNSSYPPLLRPERKADLIIHLNYCAGSQTKPLKQTCEYCTVQNIPFPKYELPDENENLKECYLMENPQEPDAPIVTFFPLINDTFRKYKAPGVERSPEELEQGQVDIYGPKTPYATKELTYTEATFDKLVKLSEYNILNNKDTLLQALRLAVEKKKRLKGQCPS

• Full Name: phospholipase A2, group IVE
• Calculated Molecular Weight: 868 aa, 99 kDa
• Observed Molecular Weight: 99 kDa, 57 kDa
• GenBank Accession Number: BC101584
• Gene Symbol: PLA2G4E
• Gene ID (NCBI): 123745
• RRID: AB_10859777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3MJ16
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).