PLAGL2 Monoclonal antibody

PLAGL2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C2E5

Cat no : 68606-1-Ig

Synonyms

FLJ23283, KIAA0198, PLAGL2, Zinc finger protein PLAGL2

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Validation Data Gallery

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  WB analysis using 68606-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of rabbit testis using 68606-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Caco-2 cells, rabbit testis tissue, Caco-2. COLO320, HepG2, MOLT-4, Ramos, HL-60

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68606-1-Ig targets PLAGL2 in WB, ELISA applications and shows reactivity with Human, rabbit samples.

• Tested Reactivity: Human, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLAGL2 fusion protein Ag30227
• Full Name: pleiomorphic adenoma gene-like 2
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC023655
• Gene Symbol: PLAGL2
• Gene ID (NCBI): 5326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).