PLAGL2 Monoclonal antibody, PBS Only

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  WB analysis using 68606-1-Ig (same clone as 68606-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit testis using 68606-1-Ig (same clone as 68606-1-PBS)

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 68606-1-Ig (same clone as 68606-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PLAGL2 Monoclonal antibody (68606-1-Ig, Clone:3C2E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

PLAGL2 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68606-1-PBS

Clone No.3C2E5

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, Indirect ELISA

Synonyms

ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5

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Product Information

68606-1-PBS targets PLAGL2 in WB, Indirect ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30227

  Product name: Recombinant human PLAGL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 194-496 aa of BC023655

  Sequence: DHCDRRFYTRKDVRRHLVVHTGRKDFLCQYCAQRFGRKDHLTRHVKKSHSQELLKIKTEPVDMLGLLSCSSTVSVKEELSPVLCMASRDVMGTKAFPGMLPMGMYGAHIPTMPSTGVPHSLVHNTLPMGMSYPLESSPISSPAQLPPKYQLGSTSYLPDKLPKVEVDSFLAELPGSLSLSSAEPQPASPQPAAAAALLDEALLAKSPANLSEALCAANVDFSHLLGFLPLNLPPCNPPGATGGLVMGYSQAEAQPLLTTLQAQPQDSPGAGGPLNFGPLHSLPPVFTSGLSSTTLPRFHQAFQ

• Full Name: pleiomorphic adenoma gene-like 2
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC023655
• Gene Symbol: PLAGL2
• Gene ID (NCBI): 5326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UPG8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).