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PLAP Monoclonal antibody

PLAP Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2B7C9

Cat no : 60294-1-Ig

Synonyms

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1



Tested Applications

Positive WB detected inHepG2 cells, HepG2
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

60294-1-Ig targets PLAP in WB, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLAP fusion protein Ag13361
Full Name alkaline phosphatase, placental (Regan isozyme)
Calculated molecular weight 58 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009647
Gene symbol ALPP
Gene ID (NCBI) 250
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Protocols

Product Specific Protocols
WB protocol for PLAP antibody 60294-1-IgDownload protocol
IHC protocol for PLAP antibody 60294-1-IgDownload protocol
FC protocol for PLAP antibody 60294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

J Cell Mol Med

Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs.

Authors - Xin Zhao