Validation Data Gallery
|Positive WB detected in||HepG2 cells, HepG2|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60294-1-Ig targets PLAP in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||PLAP fusion protein Ag13361|
|Full Name||alkaline phosphatase, placental (Regan isozyme)|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC009647|
|Gene ID (NCBI)||250|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
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