PLAP Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PLAP Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7C9

Cat no : 60294-1-PBS

Synonyms

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

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Validation Data Gallery

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  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PLAP (60294-1-Ig, Clone:2B7C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60294-1-PBS targets PLAP in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLAP fusion protein Ag13361
• Full Name: alkaline phosphatase, placental (Regan isozyme)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC009647
• Gene Symbol: ALPP
• Gene ID (NCBI): 250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.