PLCB2 Polyclonal antibody

PLCB2 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27456-1-AP

Synonyms

Phospholipase C beta 2, phospholipase C, beta 2, PLC beta 2, PLCB2

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Validation Data Gallery

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  WB analysis using 27456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 27456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PLCB2 antibody (27456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells
• Positive IF detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27456-1-AP targets PLCB2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCB2 fusion protein Ag25771
• Full Name: phospholipase C, beta 2
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC000939
• Gene Symbol: PLCB2
• Gene ID (NCBI): 5330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCB2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2) is also named as PLC-beta-2. PLCB2 is a critical regulator of platelet responses upon activation (PMID: 27465150). NF-κB regulates expression of PLC-β2. The PLCB2 genes was found to be differentially expressed in human breast cancer MCF-7 cells, and to be associated with multidrug resistance (PMID: 2951275). The knockdown of PLCB2 suppressed cell viability and promoted cell apoptosis by activating the Ras/Raf/MAPK pathway. Thus, PLCB2 may utilized as a potential therapeutic target in patients with melanoma (PMID: 31746389).