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PLEKHF1 Polyclonal antibody

PLEKHF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20389-1-AP

Synonyms

Apoptosis inducing protein, APPD, LAPF, Phafin 1, PHAFIN1, PLEKHF1, ZFYVE15



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human kidney tissue, mouse lung tissue, mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20389-1-AP is PLEKHF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHF1 fusion protein Ag14228
Full Name pleckstrin homology domain containing, family F (with FYVE domain) member 1
Calculated molecular weight 279 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC002744
Gene symbol PLEKHF1
Gene ID (NCBI) 79156
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.

Protocols

Product Specific Protocols
WB protocol for PLEKHF1 antibody 20389-1-APDownload protocol
IHC protocol for PLEKHF1 antibody 20389-1-APDownload protocol
IF protocol for PLEKHF1 antibody 20389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Breast Cancer Res

Functional characterization of the 19q12 amplicon in grade III breast cancers.

Authors - Natrajan Rachael R
  • KD Validated