Plasminogen Monoclonal antibody

Plasminogen Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A11

Cat no : 66399-1-Ig

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66399-1-Ig targets Plasminogen in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Plasminogen fusion protein Ag9938
Full Name plasminogen
Calculated Molecular Weight 810 aa, 91 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC060513
Gene Symbol Plasminogen
Gene ID (NCBI) 5340
RRIDAB_2881773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Protocols

Product Specific Protocols
WB protocol for Plasminogen antibody 66399-1-IgDownload protocol
IHC protocol for Plasminogen antibody 66399-1-IgDownload protocol
IF protocol for Plasminogen antibody 66399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Angiogenesis

PDGFD switches on stem cell endothelial commitment.

Authors - Weisi Lu
humanWB

Aging (Albany NY)

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Authors - Pingping Ma
mouseWB

Front Cell Dev Biol

Elevated Levels of miR-144-3p Induce Cholinergic Degeneration by Impairing the Maturation of NGF in Alzheimer's Disease.

Authors - Lan-Ting Zhou
mouseIHC

Biomed Pharmacother

Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia.

Authors - Lina Wei
humanWB,IHC

Clin Transl Oncol

A prognostic gene signature for gastric cancer and the immune infiltration-associated mechanism underlying the signature gene, PLG

Authors - Hui Shi