CoraLite®594-conjugated Plasminogen Monoclonal antibody

Plasminogen Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F6A11

Cat no : CL594-66399

Synonyms

Angiostatin, DKFZp779M0222, plasminogen, PLG

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-66399

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Plasminogen antibody (CL594-66399, Clone: 1F6A11 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66399

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Plasminogen antibody (CL594-66399, Clone: 1F6A11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66399 targets Plasminogen in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasminogen fusion protein Ag9938
• Full Name: plasminogen
• Calculated Molecular Weight: 810 aa, 91 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: PLG
• Gene ID (NCBI): 5340
• RRID: AB_2919985
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.