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Cyclophilin A Polyclonal antibody
Cyclophilin A Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 10720-1-AP
|Positive WB detected in||HeLa cells, C6 cells, NIH/3T3 cells, K-562 cells, HepG2 cells, Raji cells|
|Positive IHC detected in||human colon cancer tissue, human tonsillitis tissue, human kidney tissue, human placenta tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10720-1-AP targets Cyclophilin A in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclophilin A fusion protein Ag1120|
|Full Name||peptidylprolyl isomerase A (cyclophilin A)|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC005320|
|Gene ID (NCBI)||5478|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.
|Product Specific Protocols|
|WB protocol for Cyclophilin A antibody 10720-1-AP||Download protocol|
|IHC protocol for Cyclophilin A antibody 10720-1-AP||Download protocol|
|IF protocol for Cyclophilin A antibody 10720-1-AP||Download protocol|
|FC protocol for Cyclophilin A antibody 10720-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exosomal cyclophilin A as a novel noninvasive biomarker for Epstein-Barr virus associated nasopharyngeal carcinoma.
Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.
J Med Virol
Proteomic analysis of hepatitis B surface antigen positive transgenic mouse liver and decrease of cyclophilin A.
Downregulation of Peptidylprolyl isomerase A promotes cell death and enhances doxorubicin-induced apoptosis in hepatocellular carcinoma.
Increased expression of peroxiredoxin 6 and cyclophilin A in squamous cell carcinoma of the tongue.
Biochem Biophys Res Commun