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Cyclophilin A Polyclonal antibody

Cyclophilin A Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10720-1-AP

Synonyms

Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A



Tested Applications

Positive WB detected inHeLa cells, C6 cells, NIH/3T3 cells, K-562 cells, HepG2 cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, human tonsillitis tissue, human kidney tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10720-1-AP targets Cyclophilin A in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclophilin A fusion protein Ag1120
Full Name peptidylprolyl isomerase A (cyclophilin A)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC005320
Gene symbol PPIA
Gene ID (NCBI) 5478
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

Protocols

Product Specific Protocols
WB protocol for Cyclophilin A antibody 10720-1-APDownload protocol
IHC protocol for Cyclophilin A antibody 10720-1-APDownload protocol
IF protocol for Cyclophilin A antibody 10720-1-APDownload protocol
FC protocol for Cyclophilin A antibody 10720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Exosomal cyclophilin A as a novel noninvasive biomarker for Epstein-Barr virus associated nasopharyngeal carcinoma.

Authors - Lingzhi Liu
humanWB

Oncotarget

Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.

Authors - Ya Tao
human,mouseWB

J Med Virol

Proteomic analysis of hepatitis B surface antigen positive transgenic mouse liver and decrease of cyclophilin A.

Authors - Chao Zhao

Gene

Downregulation of Peptidylprolyl isomerase A promotes cell death and enhances doxorubicin-induced apoptosis in hepatocellular carcinoma.

Authors - Shaobing Cheng
humanIHC

Oral Dis

Increased expression of peroxiredoxin 6 and cyclophilin A in squamous cell carcinoma of the tongue.

Authors - Huang C-F CF
humanWB

Biochem Biophys Res Commun

PMID: 25767076

Authors - Ping Wu