PPP1R12C Polyclonal antibody

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  WB analysis using 25157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25157-1-AP (PPP1R12C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25157-1-AP (PPP1R12C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 25157-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 25157-1-AP (PPP1R12C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PPP1R12C Polyclonal Antibody for WB, ELISA

Cat No. 25157-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LENG3, MBS85, p84, Protein phosphatase 1 myosin-binding subunit of 85 kDa, Protein phosphatase 1 myosin-binding subunit p85

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Tested Applications

• Positive WB detected in: mouse colon tissue, HEK-293T cells, mouse heart tissue, rat heart tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25157-1-AP targets PPP1R12C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18559

  Product name: Recombinant human PPP1R12C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 422-716 aa of BC008030

  Sequence: GLLKTGSSGALGPPERRIAEGAPGAGLQRSASSSWLEGTSTQAKELRLARITPTPSPKLPEPSVLSEVTKPPPCLENSSPPSRIPEPESPAKPNVPTASTAPPADSRDRRRSYQMPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAEKAAGKAPESEKPAQSLDPSRRPRVPGVENSDSPAQREAPDGQGPGPQAAREHRKVGKEWRGPAEGEEAEPADRSQESSTLEGGPSARRQRWQRDLNPEPEPESEEPDGGFRTLYAELRRENERLREALTETTLRLAQLKVELE

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 12C
• Calculated Molecular Weight: 782 aa, 85 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC008030
• Gene Symbol: PPP1R12C
• Gene ID (NCBI): 54776
• RRID: AB_3669464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZL4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R12C is a subunit of myosin phosphatase. PPP1R12C regulates the catalytic activity of protein phosphatase 1 delta and assembly of the actin cytoskeleton.