PPP2R2B Monoclonal antibody

PPP2R2B Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E1D5

Cat no : 67783-1-Ig

Synonyms

B55 BETA, FLJ95686, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2B, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12



Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67783-1-Ig targets PPP2R2B in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP2R2B fusion protein Ag30774
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
Calculated molecular weight 443 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC031790
Gene symbol PPP2R2B
Gene ID (NCBI) 5521
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PPP2R2B antibody 67783-1-IgDownload protocol
IHC protocol for PPP2R2B antibody 67783-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols