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CoraLite® Plus 488-conjugated PPP2R2B Monoclonal antibody

PPP2R2B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E1D5

Cat no : CL488-67783

Synonyms

B55 BETA, FLJ95686, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2B, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PPP2R2B Monoclonal a (CL488-67783)

    FC experiment of HeLa using CL488-67783

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2R2B (CL488-67783, Clone:2E1D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67783 targets PPP2R2B in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP2R2B fusion protein Ag30774
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
Calculated Molecular Weight 443 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC031790
Gene Symbol PPP2R2B
Gene ID (NCBI) 5521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PPP2R2B antibody CL488-67783Download protocol
Standard Protocols
Click here to view our Standard Protocols