EPCR/CD201 Polyclonal antibody

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  WB analysis using 32134-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 32134-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 32134-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 32134-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32134-1-AP (EPCR/CD201 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCR/CD201 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32134-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Activated protein C receptor, APC receptor, CD201, Centrocyclin, Centrosomal protein CCD41

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Tested Applications

• Positive WB detected in: THP-1 cells, bEnd.3 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32134-1-AP targets EPCR/CD201 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: protein C receptor, endothelial
• Calculated Molecular Weight: 27kDa
• Observed Molecular Weight: 27 kDa, 40-50 kDa
• GenBank Accession Number: NM_011171.2
• Gene Symbol: PROCR
• Gene ID (NCBI): 19124
• RRID: AB_3670204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q64695
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424). EPCR is approximately 46 kDa, truncated EPCR is 23 kDa, truncated EPCR with the transmembrane domain is 26 kDa, and soluble EPCR is 43 kDa (PMID: 11686350).