PRTN3 Polyclonal antibody

PRTN3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27153-1-AP

Synonyms

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

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Validation Data Gallery

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  WB analysis of human spleen using 27153-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27153-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27153-1-AP (PRTN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27153-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27153-1-AP (PRTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human spleen tissue, human placenta tissue
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27153-1-AP is PRTN3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRTN3 fusion protein Ag25915
• Full Name: proteinase 3
• Calculated Molecular Weight: 256 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC096183
• Gene Symbol: PRTN3
• Gene ID (NCBI): 5657
• RRID: AB_2880777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.