PRUNE Polyclonal antibody, PBS Only

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• PRUNE Polyclonal antibody, PBS Only (18537-1-PBS)

• 

  WB analysis using 18537-1-AP (same clone as 18537-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  WB result of PRUNE antibody (18537-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRUNE transfected HepG2 cells. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  IP result of anti-PRUNE (IP:18537-1-AP, 3ug; Detection:18537-1-AP 1:500) with HepG2 cells lysate 1720ug. This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18537-1-AP (same clone as 18537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18537-1-AP (same clone as 18537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18537-1-AP (same clone as 18537-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRUNE antibody (18537-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18537-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PRUNE Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18537-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Exopolyphosphatase PRUNE1, EC:3.6.1.1, Drosophila-related expressed sequence 17, DRES-17, DRES17

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Product Information

18537-1-PBS targets PRUNE in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13275

  Product name: Recombinant human PRUNE protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 103-453 aa of BC025304

  Sequence: ILVDHHILSKSDTALEEAVAEVLDHRPIEPKHCPPCHVSVELVGSCATLVTERILQGAPEILDRQTAALLHGTIILDCVNMDLKIGKATPKDSKYVEKLEALFPDLPKRNDIFDSLQKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISAIYMDLEAFLQRSNLLADLHAFCQAHSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSPPLKLTPASSTHPNLHAYLQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASNSLISGLSQDEEDPPLPPTPMNSLVDECPLDQGLPKLSAEAVFEKCSQISLSQSTTASLSKK

• Full Name: prune homolog (Drosophila)
• Calculated Molecular Weight: 453 aa, 50 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC025304
• Gene Symbol: PRUNE
• Gene ID (NCBI): 58497
• RRID: AB_2172338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TP1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.