A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26922-1-AP
WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.
WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.
WB analysis of Jurkat using 26922-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 26922-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26922-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26922-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
MCF-7 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 26922-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 26922-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 26922-1-AP
IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.
IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.
IHC staining of human breast cancer using 26922-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26922-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 26922-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A549 using 26922-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PSMD9 antibody (26922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PSMD9 antibody (26922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 26922-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
U2OS cells, A549 cells
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
26922-1-AP targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.
A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26922-1-AP
WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.
WB analysis of Jurkat using 26922-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 26922-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26922-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26922-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 26922-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 26922-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 26922-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26922-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of A549 using 26922-1-AP
IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.
IF/ICC Figures
IF Staining of U2OS using 26922-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A549 using 26922-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PSMD9 antibody (26922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC (INTRA) Figures
FC experiment of HeLa using 26922-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
PSMD9 Polyclonal antibody
Catalog Number
26922-1-AP
Citations
-
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
