CoraLite® Plus 488-conjugated PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2B4

Cat no : CL488-67000

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

---

Validation Data Gallery

• 

  IF Staining of human kidney using CL488-67000

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 PTPRO antibody (CL488-67000, Clone: 2F2B4 ) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67000 targets PTPRO in IF applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTPRO fusion protein Ag8284
• Full Name: protein tyrosine phosphatase, receptor type, O
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 160 kDa, 180-220 kDa
• GenBank Accession Number: BC035960
• Gene Symbol: PTPRO
• Gene ID (NCBI): 5800
• RRID: AB_2919402
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).