Tested Applications
| Positive WB detected in | pig kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67000-1-Ig targets PTPRO in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8284 Product name: Recombinant human PTPRO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 298-597 aa of BC035960 Sequence: YWWDSASAAPESEDEFVSVLPMEYENNSTLSETEKSTSGSFSFFPVQMILTWLPPKPPTAFDGFHIHIEREENFTEYLMVDEEAHEFVAELKEPGKYKLSVTTFSSSGSCETRKSQSAKSLSFYISPSGEWIEELTEKPQHVSVHVLSSTTALMSWTSSQENYNSTIVSVVSLTCQKQKESQRLEKQYCTQVNSSKPIIENLVPGAQYQVVIYLRKGPLIGPPSDPVTFAIVPTGIKDLMLYPLGPTAVVLSWTRPYLGVFRKYVVEMFYFNPATMTSEWTTYYEIAATVSLTASVVIFP Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, O |
| Calculated Molecular Weight | 138 kDa |
| Observed Molecular Weight | 160 kDa, 180-220 kDa |
| GenBank Accession Number | BC035960 |
| Gene Symbol | PTPRO |
| Gene ID (NCBI) | 5800 |
| RRID | AB_2882317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTPRO antibody 67000-1-Ig | Download protocol |
| IHC protocol for PTPRO antibody 67000-1-Ig | Download protocol |
| WB protocol for PTPRO antibody 67000-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PTPRO (Protein tyrosine phosphatase receptor type O), catalog number 67000-1-Ig, has 5 total citations. These appear in Cell Mol Life Sci, Mediators Inflamm, Sci Rep, iScience, and Pediatr Nephrol. The research fields span neurodegeneration (Parkinson's disease), oncology (colorectal cancer, breast cancer metastasis), neuroscience (epilepsy), and nephrology (nail-patella syndrome). Applications include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci Protein tyrosine phosphatase receptor type O serves as a key regulator of insulin resistance-induced α-synuclein aggregation in Parkinson's disease
| ||
Mediators Inflamm MiR-6803-5p Promotes Cancer Cell Proliferation and Invasion via PTPRO/NF-κB Axis in Colorectal Cancer. | ||
iScience Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment | ||
Pediatr Nephrol Clinicopathological characteristics and cyclosporine-responsive proteinuria in nail-patella syndrome with a novel LMX1B mutation. |
