PYCRL Monoclonal antibody

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  WB analysis using 68087-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68087-1-Ig (PYCRL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit kidney using 68087-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68087-1-Ig (PYCRL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 68087-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PYCRL antibody (68087-1-Ig, Clone: 1G7A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PYCRL Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68087-1-Ig

Clone No.1G7A3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

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Tested Applications

• Positive WB detected in: HeLa cells, rabbit kidney tissue, HEK293 cells, LNCaP cells, MCF-7 cells, U2OS cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

68087-1-Ig targets PYCRL in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PYCRL fusion protein Ag31938
• Full Name: pyrroline-5-carboxylate reductase-like
• Calculated Molecular Weight: 274 aa, 29 kDa
• Observed Molecular Weight: 26-29 kDa
• GenBank Accession Number: BC007993
• Gene Symbol: PYCRL
• Gene ID (NCBI): 65263
• RRID: AB_2918824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q53H96
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Discov; Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer; Authors - Tina Becirovic ; KD Validated