Phospho-ASK1 (Ser966) Recombinant monoclonal antibody

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  WB analysis of COS-7 using 80142-8-RR

  Non-treated COS-7, phosphatase inhibitor treated and λ phosphatase treated COS-7 cells were subjected to SDS PAGE followed by western blot with 80142-8-RR (Phospho-ASK1 (Ser966) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  WB analysis using 80142-8-RR

  NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80142-8-RR (Phospho-ASK1 (Ser966) antibody) blocked with BSA only, Phospho-ASK1 (Ser966) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-ASK1 (Ser966) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 80142-8-RR

Clone No.252570F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, ELISA

Synonyms

ASK1 (Ser966), ASK1 (Ser967), p-ASK1, p-ASK1 (Ser966), p-ASK1 (Ser967)

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Tested Applications

• Positive WB detected in: COS-7 cells, NIH/3T3 cells, λ phosphatase treated COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80142-8-RR targets Phospho-ASK1 (Ser966) in WB, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase kinase kinase 5
• Calculated Molecular Weight: 155 kDa
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: BC054503
• Gene Symbol: ASK1
• Gene ID (NCBI): 4217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99683
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. The predicted MW of ASK1 is around 155 kDa and the 110-120 kDa fragment has also been reported.