Phospho-LYN (Tyr508) Recombinant monoclonal antibody

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  WB analysis using 86947-2-RR

  Ramos cell lysates were subjected to SDS PAGE followed by western blot with 80351-3-RR (Phospho-LYN (Tyr508)antibody) blocked with BSA only, Phospho-LYN (Tyr508) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Phospho-LYN (Tyr508) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86947-2-RR

Clone No.251104F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LYN, EC:2.7.10.2, JTK8, Lck/Yes-related novel protein tyrosine kinase, Tyrosine protein kinase Lyn

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Tested Applications

• Positive WB detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86947-2-RR targets Phospho-LYN (Tyr508) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Calculated Molecular Weight: 512 aa, 59 kDa
• Observed Molecular Weight: 53 kDa, 56 kDa
• GenBank Accession Number: BC075002
• Gene Symbol: LYN
• Gene ID (NCBI): 4067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07948
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The C-terminal tyrosine-508 residue (Tyr-508) in Lyn or the corresponding conserved tyrosine residue in each of the other members of the SFK family is the primary site for regulating tyrosine kinase activity by reversible phosphorylation. Phosphorylation at the tyrosine residue induces a closed conformation that is enzymatically inactive. (PMID: 33004926)