Product Information
28953-1-PBS targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | serine/threonine kinase 4 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 52-56 kDa |
| GenBank Accession Number | BC005231 |
| Gene Symbol | MST1 |
| Gene ID (NCBI) | 6789 |
| RRID | AB_2918221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13043 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.
