Phospho-TAU (Ser396/404) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87061-1-RR (same clone as 87061-1-PBS)

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87061-1-RR (Phospho-TAU(Ser396/404) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87061-1-PBS in a different storage buffer formulation.

Phospho-TAU (Ser396/404) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87061-1-PBS

Clone No.252396E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

p TAU, p TAU (Ser396/404), p TAU Ser396/404, p -TAU Ser396/404, p -TAU -Ser396/404

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Product Information

87061-1-PBS targets Phospho-TAU (Ser396/404) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tau isolated from individuals with AD is extensively phosphorylated and more than 40 sites have been reported so far. Hyperphosphorylated tau has an impaired ability to stabilize microtubules, and is capable of self-assembling into aggregates that can template normal tau into filaments, making hyperphosphorylation a clear disease-determining posttranslational modification in tauopathies.