Phospho-TAU (Ser396/404) Recombinant monoclonal antibody

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  WB analysis using 87061-1-RR

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87061-1-RR (Phospho-TAU(Ser396/404) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-TAU (Ser396/404) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87061-1-RR

Clone No.252396E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

p TAU, p TAU (Ser396/404), p TAU Ser396/404, p -TAU Ser396/404, p -TAU -Ser396/404

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87061-1-RR targets Phospho-TAU (Ser396/404) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau isolated from individuals with AD is extensively phosphorylated and more than 40 sites have been reported so far. Hyperphosphorylated tau has an impaired ability to stabilize microtubules, and is capable of self-assembling into aggregates that can template normal tau into filaments, making hyperphosphorylation a clear disease-determining posttranslational modification in tauopathies.