Piccolo Polyclonal antibody, PBS Only

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• Piccolo Polyclonal antibody, PBS Only (18156-1-PBS)

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  IF Staining of mouse brain using 18156-1-AP (same clone as 18156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18156-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 18156-1-AP (same clone as 18156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18156-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18156-1-AP (same clone as 18156-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18156-1-PBS in a different storage buffer formulation.

Piccolo Polyclonal Antibody for IF-P, Indirect ELISA

Cat No. 18156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, Indirect ELISA

Synonyms

ACZ, Aczonin, KIAA0559, PCLO, Protein piccolo

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Product Information

18156-1-PBS targets Piccolo in IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12952

  Product name: Recombinant human PCLO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-356 aa of BC001304

  Sequence: MKKFRVSLVSKVGKQKYVDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTAHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

• Full Name: piccolo (presynaptic cytomatrix protein)
• Calculated Molecular Weight: 553 kDa
• GenBank Accession Number: BC001304
• Gene Symbol: Piccolo
• Gene ID (NCBI): 27445
• RRID: AB_3085556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6V0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).