Piccolo Polyclonal antibody

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  IF Staining of mouse brain using 18156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 18156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Piccolo Polyclonal Antibody for IF-P, ELISA

Cat No. 18156-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, ELISA

Synonyms

ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18156-1-AP targets Piccolo in IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12952

  Product name: Recombinant human PCLO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-356 aa of BC001304

  Sequence: MKKFRVSLVSKVGKQKYVDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTAHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

• Full Name: piccolo (presynaptic cytomatrix protein)
• Calculated Molecular Weight: 553 kDa
• GenBank Accession Number: BC001304
• Gene Symbol: Piccolo
• Gene ID (NCBI): 27445
• RRID: AB_3085556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6V0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).