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Piccolo Polyclonal antibody

Piccolo Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 18156-1-AP

Synonyms

ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18156-1-AP targets Piccolo in IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Piccolo fusion protein Ag12952
Full Name piccolo (presynaptic cytomatrix protein)
Calculated Molecular Weight 553 kDa
GenBank Accession NumberBC001304
Gene Symbol PCLO
Gene ID (NCBI) 27445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).

Protocols

Product Specific Protocols
IF protocol for Piccolo antibody 18156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols