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  • KD/KO Validated

RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 11671-1-AP

Synonyms

RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein



Tested Applications

Positive WB detected inU-251 cells, human brain tissue, NIH/3T3 cells, U2OS cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11671-1-AP targets RAB1A in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB1A fusion protein Ag2264
Full Name RAB1A, member RAS oncogene family
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC000905
Gene symbol RAB1A
Gene ID (NCBI) 5861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Protocols

Product Specific Protocols
WB protocol for RAB1A antibody 11671-1-APDownload protocol
IHC protocol for RAB1A antibody 11671-1-APDownload protocol
IF protocol for RAB1A antibody 11671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Rep

Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.

Authors - Zhengwu Cheng
  • KD Validated
humanWB,IP,IF

Autophagy

RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.

Authors - Xianming Ding
  • KO Validated
mouseWB,IF

Cell Rep

Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.

Authors - Xin Zhang
  • KO Validated
humanIHC

Biomarkers

Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.

Authors - Dominik Andre Megger
humanWB

Oncol Rep

Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells.

Authors - Hui Xu
  • KD Validated
humanIHC,WB

Future Oncol

RAB1A regulates glioma cellular proliferation and invasion via the mTOR signaling pathway and epithelial-mesenchymal transition.

Authors - Bingcheng Ren
  • KD Validated