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- KD/KO Validated
RAB1A Polyclonal antibody
RAB1A Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 11671-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, NIH/3T3 cells, U-251 cells, human brain tissue, rat brain tissue, U2OS cells|
|Positive IHC detected in||human lung cancer tissue, human liver tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||C2C12 cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 9 publications below|
|WB||See 11 publications below|
|IHC||See 6 publications below|
|IF||See 5 publications below|
|IP||See 3 publications below|
11671-1-AP targets RAB1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB1A fusion protein Ag2264|
|Full Name||RAB1A, member RAS oncogene family|
|Calculated molecular weight||205 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC000905|
|Gene ID (NCBI)||5861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).
|Product Specific Protocols|
|WB protocol for RAB1A antibody 11671-1-AP||Download protocol|
|IHC protocol for RAB1A antibody 11671-1-AP||Download protocol|
|IF protocol for RAB1A antibody 11671-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Hum Mol Genet
Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.
Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.
Mol Cell Proteomics
Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.
Hum Mol Genet
Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.