RAB1A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB1A Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 11671-1-AP

Synonyms

RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein

---

Validation Data Gallery

• 

  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

• 

  WB analysis of NIH/3T3 using 11671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 11671-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11671-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11671-1-AP (RAB1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 11671-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB1A antibody (11671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 11671-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.5 ug Anti-Human RAB1A (11671-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, U-251 cells, human brain tissue, rat brain tissue, U2OS cells
• Positive IHC detected in: human lung cancer tissue, human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 9 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 3 publications below

Product Information

11671-1-AP targets RAB1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB1A fusion protein Ag2264
• Full Name: RAB1A, member RAS oncogene family
• Calculated molecular weight: 205 aa, 23 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC000905
• Gene symbol: RAB1A
• Gene ID (NCBI): 5861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Publications

Species	Application	Title
human	WB, IP	Cancer Cell; Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.; Authors - Janice D Thomas ; KD Validated
human	IP	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
mouse	WB	Hum Mol Genet; Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.; Authors - Gaynor A Smith
human	WB,IHC,IF	Cancer Lett; Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.; Authors - Tinglei Huang ; KD Validated
mouse	WB	Mol Cell Proteomics; Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.; Authors - Campbell-Valois François-Xavier FX
mouse	IF	Hum Mol Genet; Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.; Authors - Mukesh Gautam

Related Products

RAB4A Polyclonal antibody

10347-1-AP

GOLGA2/GM130 Polyclonal antibody

11308-1-AP

RAB5A Polyclonal antibody

11947-1-AP

RAB6A Polyclonal antibody

10187-2-AP

RAB1B Polyclonal antibody

17824-1-AP

RAB41 Polyclonal antibody

18818-1-AP