RAB1A Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB1A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

16

Cat no : 11671-1-AP

Synonyms

RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein

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Validation Data Gallery

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  WB analysis using 11671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

• 

  WB analysis of human brain using 11671-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 11671-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11671-1-AP (RAB1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11671-1-AP (RAB1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11671-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11671-1-AP (RAB1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 11671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 11671-1-AP (RAB1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: U-251 cells, human brain tissue, NIH/3T3 cells, U2OS cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 9 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Product Information

11671-1-AP targets RAB1A in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB1A fusion protein Ag2264
• Full Name: RAB1A, member RAS oncogene family
• Calculated molecular weight: 205 aa, 23 kDa
• Observed molecular weight: 23 kDa
• GenBank accession number: BC000905
• Gene symbol: RAB1A
• Gene ID (NCBI): 5861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Publications

Species	Application	Title
human	IHC	Oncol Rep; Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.; Authors - Zhengwu Cheng ; KD Validated
human	WB,IP,IF	Autophagy; RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.; Authors - Xianming Ding ; KO Validated
mouse	WB,IF	Cell Rep; Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.; Authors - Xin Zhang ; KO Validated
human	IHC	Biomarkers; Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.; Authors - Dominik Andre Megger
human	WB	Oncol Rep; Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells.; Authors - Hui Xu ; KD Validated
human	IHC,WB	Future Oncol; RAB1A regulates glioma cellular proliferation and invasion via the mTOR signaling pathway and epithelial-mesenchymal transition.; Authors - Bingcheng Ren ; KD Validated

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