|Positive WB detected in||U-251 cells, human brain tissue, NIH/3T3 cells, U2OS cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11671-1-AP targets RAB1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB1A fusion protein Ag2264|
|Full Name||RAB1A, member RAS oncogene family|
|Calculated molecular weight||205 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC000905|
|Gene ID (NCBI)||5861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).
Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.
RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.
Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.
Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.
Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells.
RAB1A regulates glioma cellular proliferation and invasion via the mTOR signaling pathway and epithelial-mesenchymal transition.