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- KD/KO Validated
RAB1A Polyclonal antibody
RAB1A Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF
Conjugate
Unconjugated
Cat no : 11671-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, human brain tissue, NIH/3T3 cells, U2OS cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 9 publications below |
WB | See 11 publications below |
IHC | See 6 publications below |
IF | See 4 publications below |
IP | See 3 publications below |
Product Information
11671-1-AP targets RAB1A in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RAB1A fusion protein Ag2264 |
Full Name | RAB1A, member RAS oncogene family |
Calculated molecular weight | 205 aa, 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC000905 |
Gene symbol | RAB1A |
Gene ID (NCBI) | 5861 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).
Protocols
Product Specific Protocols | |
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WB protocol for RAB1A antibody 11671-1-AP | Download protocol |
IHC protocol for RAB1A antibody 11671-1-AP | Download protocol |
IF protocol for RAB1A antibody 11671-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncol Rep Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.
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Autophagy RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.
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Cell Rep Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.
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Biomarkers Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags. | ||
Oncol Rep Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells.
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Future Oncol RAB1A regulates glioma cellular proliferation and invasion via the mTOR signaling pathway and epithelial-mesenchymal transition.
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