- Featured Product
- KD/KO Validated
RAB1A Polyclonal antibody
RAB1A Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 11671-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-251 cells, human brain tissue, NIH/3T3 cells, U2OS cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 3 publications below |
Product Information
11671-1-AP targets RAB1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB1A fusion protein Ag2264 |
| Full Name | RAB1A, member RAS oncogene family |
| Calculated molecular weight | 205 aa, 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC000905 |
| Gene symbol | RAB1A |
| Gene ID (NCBI) | 5861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB1A antibody 11671-1-AP | Download protocol |
| IHC protocol for RAB1A antibody 11671-1-AP | Download protocol |
| IF protocol for RAB1A antibody 11671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Rep Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer.
| ||
Autophagy RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells.
| ||
Cell Rep Amino acids-Rab1A-mTORC1 signaling controls whole-body glucose homeostasis.
| ||
Biomarkers Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags. | ||
Oncol Rep Inhibition of RAB1A suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells.
| ||
Biochem Biophys Res Commun CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p. |
