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  • KD/KO Validated

RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11671-1-AP

Synonyms

RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, NIH/3T3 cells, U-251 cells, rat brain tissue, U2OS cells
Positive IP detected inA549 cells, C2C12 cells
Positive IHC detected inhuman lung cancer tissue, human liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, NIH/3T3 cells, A549 cells
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11671-1-AP targets RAB1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB1A fusion protein Ag2264
Full Name RAB1A, member RAS oncogene family
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC000905
Gene symbol RAB1A
Gene ID (NCBI) 5861
RRIDAB_2173437
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

Protocols

Product Specific Protocols
WB protocol for RAB1A antibody 11671-1-APDownload protocol
IHC protocol for RAB1A antibody 11671-1-APDownload protocol
IF protocol for RAB1A antibody 11671-1-APDownload protocol
IP protocol for RAB1A antibody 11671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP

Cancer Cell

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Authors - Janice D Thomas
  • KD Validated
humanIP

EMBO J

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

Authors - Chantal Sellier
mouseWB

Hum Mol Genet

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Authors - Gaynor A Smith
humanWB,IHC,IF

Cancer Lett

Rab1A promotes IL-4R/JAK1/STAT6-dependent metastasis and determines JAK1 inhibitor sensitivity in non-small cell lung cancer.

Authors - Tinglei Huang
  • KD Validated
mouseWB

Mol Cell Proteomics

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Authors - Campbell-Valois François-Xavier FX
mouseIF

Hum Mol Genet

Absence of alsin function leads to corticospinal motor neuron vulnerability via novel disease mechanisms.

Authors - Mukesh Gautam

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (02-22-2024)

recognized a band around the expected MW, but this antibody gave a dirty background.

  • Applications: Western Blot