RAB27B Monoclonal antibody

• Featured Product
• KD/KO Validated

RAB27B Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2B9G9

Cat no : 66944-1-Ig

Synonyms

C25KG, RAB27B, Ras related protein Rab 27B

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Validation Data Gallery

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  WB analysis of HeLa using 66944-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66944-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66944-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 66944-1-Ig

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB27B (66944-1-Ig, Clone:2B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

66944-1-Ig targets RAB27B in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB27B fusion protein Ag18756
• Full Name: RAB27B, member RAS oncogene family
• Calculated Molecular Weight: 218 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC027474
• Gene Symbol: RAB27B
• Gene ID (NCBI): 5874
• RRID: AB_2882268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB,IF	J Biol Chem; The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.; Authors - Rachel Underwood ; KD Validated
mouse	IF	FEBS Open Bio; Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells.; Authors - Tomoko Kadowaki
human	WB	Am J Reprod Immunol; Rab27b promotes endometriosis by enhancing invasiveness of ESCs and promoting angiogenesis; Authors - Jing Shan