RAB27B Monoclonal antibody

RAB27B Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2B9G9

Cat no : 66944-1-Ig

Synonyms

C25KG, RAB27B, Ras related protein Rab 27B



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66944-1-Ig targets RAB27B in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB27B fusion protein Ag18756
Full Name RAB27B, member RAS oncogene family
Calculated molecular weight 218 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC027474
Gene symbol RAB27B
Gene ID (NCBI) 5874
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Protocols

Product Specific Protocols
WB protocol for RAB27B antibody 66944-1-IgDownload protocol
IHC protocol for RAB27B antibody 66944-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

FEBS Open Bio

Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells.

Authors - Tomoko Kadowaki
humanWB,IF

J Biol Chem

The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of α-synuclein.

Authors - Rachel Underwood
  • KD Validated