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  • KD/KO Validated

RAB5B Polyclonal antibody

RAB5B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27403-1-AP

Synonyms

Ras-related protein Rab-5B, Ras related protein Rab 5B, EC:3.6.5.2



Tested Applications

Positive WB detected inmouse lung tissue, Jurkat cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27403-1-AP targets RAB5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB5B fusion protein Ag26584
Full Name RAB5B, member RAS oncogene family
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC032740
Gene Symbol RAB5B
Gene ID (NCBI) 5869
RRIDAB_2880862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

Protocols

Product Specific Protocols
WB protocol for RAB5B antibody 27403-1-APDownload protocol
IF protocol for RAB5B antibody 27403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

bioRxiv

The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host

Authors - Seongok Kim
human,mouseIHC,WB,FC

Mol Hum Reprod

Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis

Authors - Hairui Fan
mouseWB

Development

A crucial role for Arf6 in the response of commissural axons to Slit.

Authors - Mariko Kinoshita-Kawada

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xin (Verified Customer) (04-15-2022)

It is ok to detect endogenous Rab5B, although the signal is a little weak.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse cortex lysate
RAB5B Antibody Western Blot validation (1:1000 dilution) in mouse cortex lysate (Cat no:27403-1-AP)