Validation Data Gallery
|Positive WB detected in||mouse lung tissue, Jurkat cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells|
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27403-1-AP targets RAB5B in WB, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB5B fusion protein Ag26584|
|Full Name||RAB5B, member RAS oncogene family|
|Calculated molecular weight||215 aa, 24 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC032740|
|Gene ID (NCBI)||5869|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xin (Verified Customer) (04-15-2022)
It is ok to detect endogenous Rab5B, although the signal is a little weak.