Multi-rAb® RAB7A Multi-Recombinant antibody

• 

  WB analysis using RMX00029

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00029 (RAB7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using RMX00029

  IP result of anti-RAB7A (IP:RMX00029, 4ug; Detection:RMX00029 1:5000) with SH-SY5Y cells lysate 1480 ug.

• 

  IP experiment of mouse brain using RMX00029

  IP result of anti-RAB7A (IP:RMX00029, 4ug; Detection:RMX00029 1:1000) with mouse brain tissue lysate 1960 ug.

• 

  IHC staining of mouse kidney using RMX00029

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00029 (RAB7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using RMX00029

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RMX00029 (RAB7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using RMX00029

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB7A antibody (RMX00029) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RAB7A Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00029

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RAB7, EC:3.6.5.2, Ras related protein Rab 7a, Ras-related protein Rab-7a

---

Tested Applications

• Positive WB detected in: A431 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue, SH-SY5Y cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00029 targets RAB7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: RAB7A, member RAS oncogene family
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: NM_004637
• Gene Symbol: RAB7A
• Gene ID (NCBI): 7879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P51149
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.