RABEP1 Monoclonal antibody, PBS Only

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  WB analysis using 68285-1-Ig (same clone as 68285-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68285-1-Ig (RABEP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68285-1-Ig (same clone as 68285-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68285-1-Ig (same clone as 68285-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68285-1-PBS in a different storage buffer formulation.

RABEP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68285-1-PBS

Clone No.1E7E11

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

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Product Information

68285-1-PBS targets RABEP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6023

  Product name: Recombinant human RABEP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 561-862 aa of BC041700

  Sequence: QLMLRQANDQLEKTMKDKQELEDFIKQSSEDSSHQISALVLRAQASEILLEELQQGLSQAKRDVQEQMAVLMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQQAEDFILPDTTEALRELVLKYREDIINVRTAADHVEEKLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIASISSLKAELERIKVEKGQLESTLREKSQQLESLQEIKISLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLTDINQLPET

• Full Name: rabaptin, RAB GTPase binding effector protein 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC041700
• Gene Symbol: RABEP1
• Gene ID (NCBI): 9135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15276
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)