RABEP1 Monoclonal antibody

RABEP1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E11

Cat no : 68285-1-Ig

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A

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Validation Data Gallery

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  WB analysis using 68285-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68285-1-Ig (RABEP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68285-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 68285-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RABEP1 antibody (68285-1-Ig, Clone: 1E7E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68285-1-Ig targets RABEP1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RABEP1 fusion protein Ag6023
• Full Name: rabaptin, RAB GTPase binding effector protein 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC041700
• Gene Symbol: RABEP1
• Gene ID (NCBI): 9135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)