Tested Applications
| Positive WB detected in | HeLa cells, SW480 cells, NCCIT cells, Daudi cells, HaCaT cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68560-1-Ig targets RAD51AP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28385 Product name: Recombinant human RAD51AP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-352 aa of BC016330 Sequence: MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELKQDKPKPNLNNLRKEEIPVQEKTPKKRLPEGTFSIPASAVPCTKMALDDKLYQRDLEVALALSVKELPTVTTNVQNSQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAAAQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKKEVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIRSPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATST Predict reactive species |
| Full Name | RAD51 associated protein 1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC016330 |
| Gene Symbol | RAD51AP1 |
| Gene ID (NCBI) | 10635 |
| RRID | AB_3085262 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96B01 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].
