Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, K-562 cells, Jurkat cells, HL-60 cells, DC2.4 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66592-1-Ig targets RAF1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||RAF1 fusion protein Ag25423|
|Full Name||v-raf-1 murine leukemia viral oncogene homolog 1|
|Calculated molecular weight||648 aa, 73 kDa|
|Observed molecular weight||70-75 kDa|
|GenBank accession number||BC018119|
|Gene ID (NCBI)||5894|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
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