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RAF1 Monoclonal antibody

RAF1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D6A1

Cat no : 66592-1-Ig

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells, HL-60 cells, DC2.4 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66592-1-Ig targets RAF1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAF1 fusion protein Ag25423
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated molecular weight 648 aa, 73 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC018119
Gene symbol RAF1
Gene ID (NCBI) 5894
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Protocols

Product Specific Protocols
WB protocol for RAF1 antibody 66592-1-IgDownload protocol
IHC protocol for RAF1 antibody 66592-1-IgDownload protocol
IF protocol for RAF1 antibody 66592-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Med Sci

Huai Qi Huang-induced Apoptosis via Down-regulating PRKCH and Inhibiting RAF/MEK/ERK Pathway in Ph+ Leukemia Cells.

Authors - Wen-Fu Xu