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RAF1 Monoclonal antibody

RAF1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse and More (2)







Cat no : 66592-1-Ig


c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

Tested Applications

Positive WB detected inHepG2 cells, DC2.4 cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

66592-1-Ig targets RAF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAF1 fusion protein Ag25423
Full Name v-raf-1 murine leukemia viral oncogene homolog 1
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC018119
Gene Symbol RAF1
Gene ID (NCBI) 5894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.


Product Specific Protocols
WB protocol for RAF1 antibody 66592-1-IgDownload protocol
IHC protocol for RAF1 antibody 66592-1-IgDownload protocol
IF protocol for RAF1 antibody 66592-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



World J Gastroenterol

TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

Authors - Cheng-Ming Guo

Cancer Cell Int

4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.

Authors - Wei Li

World J Gastrointest Oncol

Lnc524369 promotes hepatocellular carcinoma progression and predicts poor survival by activating YWHAZ-RAF1 signaling.

Authors - Wei Zheng

Curr Med Sci

Huai Qi Huang-induced Apoptosis via Down-regulating PRKCH and Inhibiting RAF/MEK/ERK Pathway in Ph+ Leukemia Cells.

Authors - Wen-Fu Xu

Cell Mol Biol Lett

Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway

Authors - Peng Liu

Sci Rep

TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway

Authors - Li Tang