RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-Ig

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

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Validation Data Gallery

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  WB analysis using 66716-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66716-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66716-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human liver cancer using 66716-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

Sample-dependent, check data in validation data gallery

Product Information

66716-1-Ig targets RBX1 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBX1 fusion protein Ag7005
• Full Name: ring-box 1
• Calculated molecular weight: 12 kDa
• Observed molecular weight: 12-15 kDa
• GenBank accession number: BC001466
• Gene symbol: RBX1
• Gene ID (NCBI): 9978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.