RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-Ig

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1



Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66716-1-Ig targets RBX1 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC001466
Gene symbol RBX1
Gene ID (NCBI) 9978
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Protocols

Product Specific Protocols
WB protocol for RBX1 antibody 66716-1-IgDownload protocol
IHC protocol for RBX1 antibody 66716-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols