RENBP Polyclonal antibody

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  WB analysis using 31209-1-AP

  U-937 cells lysates were subjected to SDS PAGE followed by western blot with 31209-1-AP (RENBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RENBP Polyclonal Antibody for WB, ELISA

Cat No. 31209-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AGE, EC:5.1.3.8, GlcNAc 2-epimerase, N-acetyl-D-glucosamine 2-epimerase, N-acylglucosamine 2-epimerase

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Tested Applications

• Positive WB detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31209-1-AP targets RENBP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RENBP fusion protein Ag35056
• Full Name: renin binding protein
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 29 kDa, 45-49 kDa
• GenBank Accession Number: BC015558
• Gene Symbol: RENBP
• Gene ID (NCBI): 5973
• RRID: AB_3669902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P51606
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Renin binding protein (RENBP) was originally identified as a protein in the kidney that was capable of binding renin in renal homogenates giving rise to a complex designated high molecular weight renin (PMID: 12866502). Studies have shown that human RnBP is the enzyme N-acetyl-D-glucosamine (GlcNAc) 2-epimerase (PMID: 12866502). RENBP has 3 isoforms produced by alternative splicing and alternative initiation with molecular weights of 29, 48, and 49 kDa, respectively.