REST Polyclonal antibody, PBS Only

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  WB analysis of Raji using 22242-1-AP (same clone as 22242-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22242-1-AP (same clone as 22242-1-PBS)

  WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22242-1-AP (same clone as 22242-1-PBS)

  IP result of anti-REST (IP:22242-1-AP, 4ug; Detection:22242-1-AP 1:15000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

REST Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 22242-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

NRSF, X2 box repressor, XBR

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Product Information

22242-1-PBS targets REST in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17707

  Product name: Recombinant human REST protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 748-1097 aa of BC132859

  Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE

• Full Name: RE1-silencing transcription factor
• Calculated Molecular Weight: 1097 aa, 122 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC132859
• Gene Symbol: REST
• Gene ID (NCBI): 5978
• RRID: AB_2879044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13127
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).