RGS12 Polyclonal antibody

RGS12 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29803-1-AP

Synonyms

DKFZp761K1617, DKFZp761K1817, RGS12

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Validation Data Gallery

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  WB analysis using 29803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 29803-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, A431 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29803-1-AP targets RGS12 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS12 fusion protein Ag30731
• Full Name: regulator of G-protein signaling 12
• Calculated Molecular Weight: 156 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_198229
• Gene Symbol: RGS12
• Gene ID (NCBI): 6002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.