Tested Applications
| Positive WB detected in | human liver tissue, HEK-293 cells, HeLa cells, L02 cells, mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue, mouse liver tissue, rat liver tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14957-1-AP targets RPS15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6795 Product name: Recombinant human RPS15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC064908 Sequence: MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK Predict reactive species |
| Full Name | ribosomal protein S15 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC064908 |
| Gene Symbol | RPS15 |
| Gene ID (NCBI) | 6209 |
| RRID | AB_2180163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62841 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS15 antibody 14957-1-AP | Download protocol |
| IHC protocol for RPS15 antibody 14957-1-AP | Download protocol |
| WB protocol for RPS15 antibody 14957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS15 Polyclonal antibody (Cat# 14957-1-AP) has 11 total citations published in journals including Nature Communications, Journal of Translational Medicine, Oncogene, Current Biology, Cell Reports, Cell Proliferation, iScience, and eLife. Research fields span ribosome biogenesis, protein translation regulation, lung cancer (small-cell and adenocarcinoma), male meiosis, leukemia survival, unfolded protein response, and drug-tolerant cancer persister cells.
| Species | Application | Title |
|---|---|---|
Nat Commun Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis. | ||
J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. | ||
J Transl Med Uridine metabolism promotes lung adenocarcinoma progression by activating FBL transcription via YBX1. | ||
Oncogene HERC4 synergizes with USP16 to promote lung adenocarcinoma growth by augmenting protein translation via stabilizing RPS15. | ||
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
Reviews
What customers say
The single review (5/5) was posted by a researcher in February 2019, who used the antibody at 1:100 dilution on HeLa cells for Western Blot and Immunofluorescence. They recommended 4% PFA fixation, blocking with BSA containing 0.05% Saponin, and overnight incubation at 4°C for optimal staining quality. Overall, the reviewer expressed strong satisfaction with the product's performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Advait (Verified Customer) (02-22-2019) | 4% PFA fixationBlocking buffer: BSA with 0.05% SaponinIncubate at 4 degrees overnight for best staining quality.
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