Tested Applications
| Positive WB detected in | human liver tissue, HeLa cells, L02 cells, mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue, mouse liver tissue, rat liver tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
14957-1-AP targets RPS15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6795 Product name: Recombinant human RPS15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC064908 Sequence: MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK Predict reactive species |
| Full Name | ribosomal protein S15 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC064908 |
| Gene Symbol | RPS15 |
| Gene ID (NCBI) | 6209 |
| RRID | AB_2180163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62841 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS15 antibody 14957-1-AP | Download protocol |
| IHC protocol for RPS15 antibody 14957-1-AP | Download protocol |
| WB protocol for RPS15 antibody 14957-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
Cell Prolif An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms | ||
Elife The molecular basis of coupling between poly(A)-tail length and translational efficiency. | ||
Elife The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1. | ||
iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Advait (Verified Customer) (02-22-2019) | 4% PFA fixationBlocking buffer: BSA with 0.05% SaponinIncubate at 4 degrees overnight for best staining quality.
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