RPS20 Polyclonal antibody

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RPS20 Polyclonal Antibody for WB, ELISA

Cat No. 15692-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

40S ribosomal protein S20, ribosomal protein S20, RPS20

• 

  WB analysis of MCF-7 using 15692-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15692-1-AP

  WB result of RPS20 antibody (15692-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS20 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 15692-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15692-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF7 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15692-1-AP targets RPS20 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8257

  Product name: Recombinant human RPS20 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-119 aa of BC007507

  Sequence: MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

• Full Name: ribosomal protein S20
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007507
• Gene Symbol: RPS20
• Gene ID (NCBI): 6224
• RRID: AB_10596626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60866
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Publications

What published studies show

The RPS20 Polyclonal antibody (Cat# 15692-1-AP) has 8 citations published in journals including Nature Cell Biology, Molecular Cell, Cell Reports, Journal of Translational Medicine, Journal of Pineal Research, Oncology Reports, and bioRxiv. Research fields span oncogenic mRNA translation specificity, ribosome-associated protein dynamics, paternal ribosome biogenesis dysfunction, radiation-induced salivary gland damage, renal clear cell carcinoma, and mitotic spindle integrity. Applications cited include WB, IHC, and IF in human and mouse samples.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity; Authors - Joanna R Kovalski
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
mouse	WB	J Transl Med; Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice.; Authors - Xin Li
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
human,mouse	WB,IHC	Oncol Rep; Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma; Authors - Cheng Shen ; KD Validated
human,mouse	WB	bioRxiv; RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages; Authors - Teodorus Theo Susanto