RPS20 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPS20 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15692-1-AP

Synonyms

40S ribosomal protein S20, ribosomal protein S20, RPS20

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 15692-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15692-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15692-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15692-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15692-1-AP (RPS20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF7 cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15692-1-AP targets RPS20 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS20 fusion protein Ag8257
• Full Name: ribosomal protein S20
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007507
• Gene Symbol: RPS20
• Gene ID (NCBI): 6224
• RRID: AB_10596626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human,mouse	WB,IHC	Oncol Rep; Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma; Authors - Cheng Shen ; KD Validated
mouse	WB	J Pineal Res; Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin; Authors - Jingming Ren
human,mouse	WB	bioRxiv; RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages; Authors - Teodorus Theo Susanto