RPS27 Polyclonal antibody

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• RPS27 Polyclonal antibody (15355-1-AP)

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• KD/KO Validated

RPS27 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15355-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MPS-1, MPS1, MPS 1, Metallopan-stimulin 1, Metallopan stimulin 1

• 

  WB analysis using 15355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15355-1-AP (RPS27 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SGC-7901 using 15355-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15355-1-AP (RPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of SGC-7901 using 15355-1-AP

  IP result of anti-RPS27 (IP:15355-1-AP, 4ug; Detection:15355-1-AP 1:500) with SGC-7901 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human endometrial cancer using 15355-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15355-1-AP (RPS27 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human endometrial cancer using 15355-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 15355-1-AP (RPS27 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15355-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS27 antibody (15355-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, SGC-7901 cells, Jurkat cells
• Positive IP detected in: SGC-7901 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15355-1-AP targets RPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7601

  Product name: Recombinant human RPS27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-84 aa of BC002658

  Sequence: MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

• Full Name: ribosomal protein S27
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC002658
• Gene Symbol: RPS27
• Gene ID (NCBI): 6232
• RRID: AB_2180509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42677
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPS27 Polyclonal antibody (Cat# 15355-1-AP) has 7 citations published in Nature Communications, Brain, Cell Reports, Cancer Metabolism, Journal of Molecular Biology, BMC Cancer, and FEBS Letters. Research fields span ribosome biogenesis, protein translation regulation, neural stem cell differentiation, lung cancer, prostate cancer metastasis, protein quality control, and Kaposi's sarcoma development.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells.; Authors - Hyun Ju Lee
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human		Cell Rep; FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.; Authors - Giorgio Ramadori
human,mouse	WB	Cancer Metab; High-fat diet promotes prostate cancer metastasis via RPS27; Authors - Dameng Li ; KD Validated
human	WB	J Mol Biol; Defective Human SRP Induces Protein Quality Control and Triggers Stress Response; Authors - Elena B Tikhonova
human	WB,IHC	BMC Cancer; Exploration of RNA-binding proteins identified RPS27 as a potential regulator associated with Kaposi's sarcoma development; Authors - Jingzhan Zhang