RRAS Polyclonal antibody

RRAS Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27457-1-AP

Synonyms

p23, Ras related protein R Ras, RRAS



Tested Applications

Positive WB detected inA549 cells, PC-3 cells, HT-29 cells
Positive IP detected inHT-29 cells
Positive IHC detected inhuman renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27457-1-AP targets RRAS in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAS fusion protein Ag26538
Full Name related RAS viral (r-ras) oncogene homolog
Calculated molecular weight 218 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC016318
Gene symbol RRAS
Gene ID (NCBI) 6237
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRAS antibody 27457-1-APDownload protocol
IHC protocol for RRAS antibody 27457-1-APDownload protocol
IP protocol for RRAS antibody 27457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Biomaterials

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Authors - Yuanyuan Liu