RRAS Polyclonal antibody
RRAS Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27457-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, PC-3 cells, HT-29 cells |
Positive IP detected in | HT-29 cells |
Positive IHC detected in | human renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
Product Information
27457-1-AP targets RRAS in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RRAS fusion protein Ag26538 |
Full Name | related RAS viral (r-ras) oncogene homolog |
Calculated molecular weight | 218 aa, 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC016318 |
Gene symbol | RRAS |
Gene ID (NCBI) | 6237 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RRAS antibody 27457-1-AP | Download protocol |
IHC protocol for RRAS antibody 27457-1-AP | Download protocol |
IP protocol for RRAS antibody 27457-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy. | ||
Transl Cancer Res Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses. | ||
Hepatology Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis |