RRM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RRM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

50

Cat no : 10526-1-AP

Synonyms

RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit

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Validation Data Gallery

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  WB analysis using 10526-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10526-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10526-1-AP

  A549 cells (shcontrol and shRNA of RRM1) were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000. .

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  WB analysis of rat skeletal muscle using 10526-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10526-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10526-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10526-1-AP

  IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.

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  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10526-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of Raji using 10526-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 10526-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RRM1 Polyclonal antibody (10526-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, A549 cells, K-562 cells, BxPC-3 cells, HEK-293 cells, U-251 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Raji cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 34 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10526-1-AP targets RRM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRM1 fusion protein Ag0789
• Full Name: ribonucleotide reductase M1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC006498
• Gene Symbol: RRM1
• Gene ID (NCBI): 6240
• RRID: AB_2180372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.

What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).

What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).

What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.

How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).

Publications

Species	Application	Title
human	WB, IF	N Engl J Med; DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.; Authors - Zhong Zheng
human	IF	Lancet Oncol; The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.; Authors - Lars Petter Jordheim
human	WB	Clin Cancer Res; PMID: 25224279; Authors - Martin Kerr ; KD Validated
human	WB, IHC, IP	Clin Cancer Res; PMID: 26228206; Authors - Francesca Vena
human	IHC	Ann Oncol; PMID: 23038758; Authors - Vilmar A C AC
	IHC	Oncotarget; Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.; Authors - Thomas J Herzog

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