CoraLite®594-conjugated SDHA Monoclonal antibody

SDHA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5E10G12

Cat no : CL594-66588

Synonyms

FP, SDH2, SDHA, SDHF



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66588-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66588 targets SDHA in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SDHA fusion protein Ag7023
Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Calculated molecular weight 73 kDa
Observed molecular weight 62-73 kDa
GenBank accession numberBC001380
Gene symbol SDHA
Gene ID (NCBI) 6389
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Protocols

Product Specific Protocols
IF protocol for CL594 SDHA antibody CL594-66588Download protocol
Standard Protocols
Click here to view our Standard Protocols