Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66588-1-Ig targets SDHA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7023 Product name: Recombinant human SDHA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 336-664 aa of BC001380 Sequence: DLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY Predict reactive species |
| Full Name | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 62-73 kDa |
| GenBank Accession Number | BC001380 |
| Gene Symbol | SDHA |
| Gene ID (NCBI) | 6389 |
| RRID | AB_2881948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31040 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SDHA antibody 66588-1-Ig | Download protocol |
| IHC protocol for SDHA antibody 66588-1-Ig | Download protocol |
| WB protocol for SDHA antibody 66588-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SDHA Monoclonal antibody (clone 5E10G12), catalog number 66588-1-Ig, has 13 citations. These appear in journals including Nature Communications, Cell Reports, Cell Chemical Biology, Advanced Materials, FASEB Journal, Biochemical Pharmacology, Cancers (Basel), and others. Research fields span mitochondrial biology, cancer (lung squamous cell carcinoma, pancreatic adenocarcinoma), cardiac disease, ferroptosis, tissue repair, renal ischemia-reperfusion injury, and antimicrobial agent development.
| Species | Application | Title |
|---|---|---|
Adv Mater A Hierarchical Plug-and-Supply Flap (PASF) for Instant Perfusion and Oxygen Delivery in Large Soft Tissue Repair. | ||
Nat Commun Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma | ||
Cell Rep Med Structure-guided development of selective caseinolytic protease P agonists as antistaphylococcal agents | ||
Sci China Life Sci Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins. | ||
Cell Chem Biol Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma. | ||
Cell Rep Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia |
Reviews
What customers say
Based on available reviews, this antibody received a 5-star rating from a user who tested it by Western Blot at a 1:10,000 dilution on mouse liver tissue. The reviewer reported very strong signal with clean background, describing it as a great antibody for WB. Overall, the feedback highlights excellent performance in terms of sensitivity and specificity for Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (07-12-2023) | Great antibody for WB. Very strong signal and clean background.
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