Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells, mouse pancreas tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15087-1-AP targets SEC61B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, monkey, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SEC61B fusion protein Ag7169|
|Full Name||Sec61 beta subunit|
|Calculated molecular weight||10 kDa|
|Observed molecular weight||10-15 kDa|
|GenBank accession number||BC001734|
|Gene ID (NCBI)||10952|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).
|Product Specific Protocols|
|WB protocol for SEC61B antibody 15087-1-AP||Download protocol|
|IHC protocol for SEC61B antibody 15087-1-AP||Download protocol|
|IF protocol for SEC61B antibody 15087-1-AP||Download protocol|
|IP protocol for SEC61B antibody 15087-1-AP||Download protocol|
|Click here to view our Standard Protocols|
QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis.
Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.
CReP mediates selective translation initiation at the endoplasmic reticulum.
J Cell Biol
Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.
Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (09-22-2020)
SEC61B Antibody have correct localization on ER.
WEI (Verified Customer) (06-29-2020)
Strong band for heart tissues, but not so sharp band.