SEC61B Polyclonal antibody

SEC61B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 15087-1-AP


Sec61 beta subunit, SEC61B

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells, mouse pancreas tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15087-1-AP targets SEC61B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC61B fusion protein Ag7169
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene symbol SEC61B
Gene ID (NCBI) 10952
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).


Product Specific Protocols
WB protocol for SEC61B antibody 15087-1-APDownload protocol
IHC protocol for SEC61B antibody 15087-1-APDownload protocol
IF protocol for SEC61B antibody 15087-1-APDownload protocol
IP protocol for SEC61B antibody 15087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.

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Genes Dev

Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wei (Verified Customer) (09-22-2020)

SEC61B Antibody have correct localization on ER.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse heart

WEI (Verified Customer) (06-29-2020)

Strong band for heart tissues, but not so sharp band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart